Comparing the 6 weeks of high-intensity interval and continuous training with standard diet on desnutrin and FNDC5 genes expression after a period of high fat diet in subcutaneous adipose tissue and the quadriceps muscle tissue of obese male rats

Introdution: Obesity is a systemic disorder that disruption the regulation of the effective proteins; in fat metabolism (Desnurtin, FNDC5 it leads to resistance to insulin and Diabetes.  The aim of this study was comparing the 6 weeks of high-intensity interval and continuous training with standard diet on desnutrin and FNDC5 genes expression in subcutaneous adipose tissue and the quadriceps muscle tissue of obese male rats. Methods: This study was fundamental, and its method was experimental. 40 Wistar rats were compared after 12 weeks of high-fat diet (n=30) and standard diet (n=10) in order to ensure obesity. Then, 30 obese rats were divided randomly into 3 groups: control (n=10), continuous training (n=10), and high-intensity interval training groups (n=10). Continues and high-intensity interval exercise trainings were conducted according to the principle of overload for 6 weeks and 6 sessions per week. Extraction and gene expression level desnutrin in subcutaneous adipose tissue, desnutrin, and FNDC5 in quadriceps muscle tissue was assessed using real time-PCR method. The one-way ANOVA test and Tukey's post hoc test were used to compare inter group changes. Results: The study showed that the difference between high-intensity interval and continuous training on desnutrin gene expression subcutaneous adipose tissue was significant (P=0.011), but there was no significant difference in desnutrin (P=0.275) and FNDC5 genes expression (P=0.981) between them in the quadriceps muscle tissue. Conclusion: For people who suffer from obesity, and for counteracting with the destructive effects of obesity on fat metabolism factors, including desnutrin and FNDC5, continuous training can be an effective method. However, for weight loss, high intensity interval training is the best method